Competitive immunoassay (Cat-EIA), a helpful technique for catalytic antibody detection. Part II

The Cat-EIA procedure, described in part I, was successfully applied to the screening of 5 different catalytic activities on a given set of 11 mAbs. The precautions required to devoid false-positive identification (preceding paper), were taken into account. Two catalytic activities were thus detecte...

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Bibliographic Details
Published inTetrahedron letters Vol. 40; no. 10; pp. 1891 - 1894
Main Authors Taran, F., Renard, P.Y., Créminon, C., Valleix, A., Frobert, Y., Pradelles, P., Grassi, J., Mioskowski, C.
Format Journal Article
LanguageEnglish
Published OXFORD Elsevier Ltd 05.03.1999
Elsevier
Subjects
Antibodies
Catalysis
Chemistry
Chemistry, Organic
Hydrolysis
Physical Sciences
Science & Technology
Thioacetal
Hydrolysis
Antibodies
Thioacetal
Catalysis
catalysis
antibodies
thioaceatal
ENZYME IMMUNOASSAYS
hydrolysis
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Summary:The Cat-EIA procedure, described in part I, was successfully applied to the screening of 5 different catalytic activities on a given set of 11 mAbs. The precautions required to devoid false-positive identification (preceding paper), were taken into account. Two catalytic activities were thus detected, including a newly thioacetal hydrolysis. The Cat-EIA technique, described in part I, was successfully used for the detection of the antibody-catalyzed hydrolyses of substrates 1–5 on a given set of 11 mAbs.
ISSN:0040-4039
1873-3581
DOI:10.1016/S0040-4039(99)00068-4