Use of planar lipid monolayers for 2D crystallisation of proteins and simple analysis of specific lipid-peptide interactions

Planar lipid monolayers provide a model system for analysing molecular mechanisms of interactions between proteins and cell membranes. Small cell lung carcinoma (SCLC) constitutes 25% of the total deaths from lung cancer. Insertion of the substance P(SP) analogues [DArg 1, Dphe 5, DTrp 7,9, Leu 11]...

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Bibliographic Details
Published inThin solid films Vol. 284; pp. 18 - 23
Main Authors Newman, R.H., Freemont, P.S.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.09.1996
Subjects
2D Crystals
Electron microscopy
Lipid protein interactions
Planar lipid monolayers
Planar lipid monolayers
Lipid protein interactions
Electron microscopy
2D Crystals
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Summary:Planar lipid monolayers provide a model system for analysing molecular mechanisms of interactions between proteins and cell membranes. Small cell lung carcinoma (SCLC) constitutes 25% of the total deaths from lung cancer. Insertion of the substance P(SP) analogues [DArg 1, Dphe 5, DTrp 7,9, Leu 11] SP and [Arg 6, DTrp 7,9], MePhe 8] SP (6–11), into phospholipid monolayers correlated with their biological activity in regulating growth in SCLC cell lines. The transmembrane helical peptides of the proto-oncogenic and oncogenic forms of the neu protein showed similar lipophilicity, suggesting receptor stabilisation without conformational change. The lipid-binding proteins annexin VI and V interacted most strongly with phosphatidylethanolamine and phosphatidylserine respectively and formed 2D crystals of trimers on phospholipid monolayers.
ISSN:0040-6090
1879-2731
DOI:10.1016/S0040-6090(96)08833-5