The role of glial and neuronal Eph/ephrin signaling in Drosophila mushroom body development and sleep and circadian behavior

• Published in: Biochemical and biophysical research communications Vol. 720; p. 150072
• Main Authors: Lee, Ji-Eun, Lee, Hyungi, Baek, Eunji, Choi, Byoungyun, Yun, Hye Sup, Yoo, Yong Kyoung, Lee, Young-Sun, Song, Gyun Jee, Cho, Kyoung Sang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.08.2024
• Subjects: Animals; Circadian Rhythm - physiology; Drosophila; Drosophila - metabolism; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Drosophila melanogaster - physiology; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Eph receptor; Ephrin ligands; Ephrins - genetics; Ephrins - metabolism; Glial cells; Mushroom Bodies - metabolism; Neurogenesis; Neuroglia - metabolism; Neurons - metabolism; Receptors, Eph Family - genetics; Receptors, Eph Family - metabolism; Signal Transduction; Sleep - genetics; Sleep - physiology; Sleep regulation; Ephrin ligands; Sleep regulation; Glial cells; Eph receptor; Neurogenesis; Drosophila
• ISSN: 0006-291X; 1090-2104; 1090-2104
• DOI: 10.1016/j.bbrc.2024.150072

The Eph receptor, a prototypically large receptor protein tyrosine kinase, interacts with ephrin ligands, forming a bidirectional signaling system that impacts diverse brain functions. Eph receptors and ephrins mediate forward and reverse signaling, affecting neurogenesis, axon guidance, and synaptic signaling. While mammalian studies have emphasized their roles in neurogenesis and synaptic plasticity, the Drosophila counterparts are less studied, especially in glial cells, despite structural similarities. Using RNAi to modulate Eph/ephrin expression in Drosophila neurons and glia, we studied their roles in brain development and sleep and circadian behavior. Knockdown of neuronal ephrin disrupted mushroom body development, while glial knockdown had minimal impact. Surprisingly, disrupting ephrin in neurons or glial cells altered sleep and circadian rhythms, indicating a direct involvement in these behaviors independent from developmental effects. Further analysis revealed distinct sleep phenotypes between neuronal and glial knockdowns, underscoring the intricate interplay within the neural circuits that govern behavior. Glia-specific knockdowns showed altered sleep patterns and reduced circadian rhythmicity, suggesting an intricate role of glia in sleep regulation. Our findings challenge simplistic models of Eph/ephrin signaling limited to neuron-glia communication and emphasize the complexity of the regulatory networks modulating behavior. Future investigations targeting specific glial subtypes will enhance our understanding of Eph/ephrin signaling's role in sleep regulation across species. •Eph/ephrin signaling in Drosophila impacts brain development & behavior.•Eph/ephrin knockdown in neurons, but not glia, disrupts mushroom body development.•Both neuronal and glial perturbations of Eph/ephrin alter sleep/circadian rhythms.•Glial knockdown reveals intricate glia-specific role in sleep regulation.