β-Galactosidase transferase activity in ice and use of vinyl-β- d-galactoside as donor

The ability of vinyl-β- d-galactoside as a donor in transglycosylation reactions catalysed by the β-glycosidase from Aspergillus oryzae using allylic alcohol and methyl-α-galactoside as acceptors is tested. A kinetic study made in comparison with another donor, the 2-nitrophenyl-β- d-galactoside, sh...

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Published inTetrahedron: asymmetry Vol. 8; no. 12; pp. 2017 - 2023
Main Authors Chiffoleau-Giraud, V., Spangenberg, P., Rabiller, C.
Format Journal Article
LanguageEnglish
Published OXFORD Elsevier Ltd 27.06.1997
Elsevier
Subjects
Chemistry
Chemistry, Inorganic & Nuclear
Chemistry, Organic
Chemistry, Physical
Physical Sciences
Science & Technology
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Summary:The ability of vinyl-β- d-galactoside as a donor in transglycosylation reactions catalysed by the β-glycosidase from Aspergillus oryzae using allylic alcohol and methyl-α-galactoside as acceptors is tested. A kinetic study made in comparison with another donor, the 2-nitrophenyl-β- d-galactoside, shows that the use of the latter leads at room temperature to better yields than those obtained from the former. A reverse situation is observed in ice at −7°C, conditions in which the yield for transglycosylation can be enhanced from 59% to 82% with methyl-α-galactoside as an acceptor. Graphic
ISSN:0957-4166
1362-511X
DOI:10.1016/S0957-4166(97)00194-8