GroEL single-ring solution structure obtained by small-angle neutron scattering

In order to study the importance of interaction between the two rings that form the GroEL cylinder, we investigated a heptameric GroEL mutant (single ring, SR) by small-angle neutron scattering. We measured the scattering curves of single rings and of complexes of single rings with GroES and ADP in...

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Published inPhysica. B, Condensed matter Vol. 276; pp. 528 - 529
Main Authors Holzinger, J, Heumann, H, Manakova, E, Rößle, M, Vanatalu, K, Wiedenmann, A, May, R.P
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.03.2000
Subjects
Biological structures
Proteins
Small-angle neutron scattering
Proteins
Biological structures
Small-angle neutron scattering
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Summary:In order to study the importance of interaction between the two rings that form the GroEL cylinder, we investigated a heptameric GroEL mutant (single ring, SR) by small-angle neutron scattering. We measured the scattering curves of single rings and of complexes of single rings with GroES and ADP in D 2O. Preliminary model calculations revealed relevant differences between the model based on the GroEL X-ray crystal structure and the experimental data.
ISSN:0921-4526
1873-2135
DOI:10.1016/S0921-4526(99)01735-4