A highly sensitive high performance liquid chromatography-electrochemical detection method for the determination of five phenolic compounds from Salvia miltiorrhiza

• Published in: Sepu Vol. 35; no. 8; p. 897
• Main Authors: Long, Zhen, Gamache, Paul, Guo, Zhimou, Pan, Yuanyuan, Zhang, Yanhai, Liu, Xiaoda, Jin, Yan, Liu, LüYe, Liang, Lina, Li, Renyong
• Format: Journal Article
• Language: Chinese
• Published: China 08.08.2017
• Subjects: Antioxidants; Caffeic Acids - analysis; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal - chemistry; Electrochemical Techniques; Phenols - analysis; Salvia miltiorrhiza - chemistry; phenolic compound; gradient elution; high performance liquid chromatography-electrochemical detection (HPLC-ECD); antioxidant; herbal medicine (HM)
• ISSN: 1000-8713
• DOI: 10.3724/SP.J.1123.2017.03038

The determination of antioxidants continues to be interested, since the oxidative damage is thought to be one of the main mechanisms involved in nearly all chronic renal pathologies. A highly sensitive high performance liquid chromatography-electrochemical detection (HPLC-ECD) method was developed for evaluating the antioxidant properties of (Dan Shen). The method was optimized with respect to selectivity and sensitivity. Chromatographic conditions, including mobile phase pH value, buffer concentration, buffer type, organic solvent type, gradient profile and flow rate, were systematically investigated. Low pH value (2.8), low buffer concentration (20 mmol/L NaH PO ), a shallow water-acetonitrile gradient, and a flow rate of 0.2 mL/min were the determined optimal conditions for the quantitative analysis of aimed five antioxidants from 14 batches of Dan Shen samples. The described method provided a good recovery (>95%), a very wide linear range (up to 10 for all analytes), a good precision (RSDs<4.01%), and a high sensitivity (LOQ of caffeic acid, 1.5 μ g/L). Compared with UV detection, the described ECD method was also more effective for evaluating the antioxidant properties of Dan Shen, as it provided highly selective detection of electro-active antioxidants.