Impact of L-Arginine on diabetes-induced neuropathy and myopathy: Roles of PAI-1, Irisin, oxidative stress, NF-κβ, autophagy and microRNA-29a
T2DM is a chronic disorder with progressive neuromuscular alterations. L-arginine (ARG) is the most common semi-essential amino acid having several metabolic functions. to investigate the impact of L-arginine in combating diabetic-induced neuromyopathy and its possible mechanisms. 24 rats were divid...
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Published in | Tissue & cell Vol. 87; p. 102342 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Scotland
Elsevier Ltd
01.04.2024
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Subjects | |
Online Access | Get full text |
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Summary: | T2DM is a chronic disorder with progressive neuromuscular alterations. L-arginine (ARG) is the most common semi-essential amino acid having several metabolic functions.
to investigate the impact of L-arginine in combating diabetic-induced neuromyopathy and its possible mechanisms.
24 rats were divided into CON, CON+ARG, DC, DC+ARG. Behavioral tests, Body weight (BW), fasting blood glucose (FBG), insulin, total antioxidant capacity (TAC), malondialdehyde (MDA), plasminogen activator inhibitor-1 (PAI-1), and irisin were done. Creatine kinase-MM (CK-MM), interleukin 4 (IL-4), interleukin 6 (IL-6), TAC, MDA, expression of microRNA-29a mRNA & light chain 3 protein were determined in muscle. Histological and NF-κβ immunohistochemical expression in muscle and nerve were assessed.
ARG supplementation to diabetic rats improved altered behavior, significantly increased BW, insulin, TAC, irisin and Il-4, decreased levels of glucose, microRNA-29a, NF-κβ and LC3 expression, PAI-1, CK-MM and restored the normal histological appearance.
ARG supplementation potently alleviated diabetic-induced neuromuscular alterations.
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•ARG improved neuromuscular abnormalities in diabetic rats.•ARG reduced inflammation and oxidative stress.•ARG reduced PAI-1, microRNA-29a, NF-κβ and LC3 expression.•ARG increased serum Irisin in diabetic rats. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0040-8166 1532-3072 |
DOI: | 10.1016/j.tice.2024.102342 |