Expanding functional spaces of enzymes by utilizing whole genome treasure for library construction

• Published in: Journal of molecular catalysis. B, Enzymatic Vol. 26; no. 3; pp. 241 - 250
• Main Authors: Oh, Hyo-Jeong, Cho, Kyoung-Won, Jung, In-Su, Kim, Won-Ho, Hur, Byung-Ki, Kim, Geun-Joong
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2003
• Subjects: Enzyme library; FSS; Functional space; Metagenome; Whole genome; Whole genome; Enzyme library; Functional space; Metagenome; FSS
• ISSN: 1381-1177; 1873-3158
• DOI: 10.1016/j.molcatb.2003.06.007

A huge database resulted from whole genome sequencings has provided a possibility of new information that is likely to extent the scope and thus changes the way of approach for the functional assigning of putative open reading frames annotated by whole genome sequence analyses. These are mainly realized by ease, one-step identification of putative genes using genomics or proteomics tools. A major challenge remained in biotechnology may translate these informations into better ways to screen or select a gene as a representative sequence. Further attempts to mine the related whole genes or partial DNA fragments from whole genome treasure, and then the incorporation of these sequences into a representative template, will result in the use of genetic information that can be translated into functional proteins or allowed the generation of new lineages as a valuable pool. Such screens enable rapid biochemical analysis and easy isolation of the target activity, thereby accelerating the screening of novel enzymes from the expanded library with related sequences. Information-based PCR amplification of whole genes and reconstitution of functional DNA fragments will provide a platform for expanding the functional spaces of potential enzymes, especially when used mixed- and metagenome as gene resources.