Immunoglobulins from sera of APS patients bind HTR-8/SVneo trophoblast cell line and reduce additional mediators of cell invasion

• Published in: Reproductive biology Vol. 17; no. 4; pp. 389 - 395
• Main Authors: Jovanović Krivokuća, Milica, Abu Rabi, Tamara, Stefanoska, Ivana, Vrzić-Petronijević, Svetlana, Petronijević, Miloš, Vićovac, Ljiljana
• Format: Journal Article
• Language: English
• Published: Poland 01.12.2017
• Subjects: Antiphospholipid Syndrome - immunology; Cell Line; Cell Movement - drug effects; Cell Movement - physiology; Cell Survival - drug effects; Cell Survival - physiology; Enzyme Inhibitors - pharmacology; Female; Humans; Imidazoles - pharmacology; Immunoglobulins - metabolism; Integrins - metabolism; Phosphorylation - drug effects; Pyridines - pharmacology; Signal Transduction - drug effects; Signal Transduction - physiology; Trophoblasts - drug effects; Trophoblasts - metabolism; Antiphospholipid antibodies; MMP-9; p38 MAPK; HTR-8/SVneo cell line; Integrins
• ISSN: 1642-431X; 2300-732X
• DOI: 10.1016/j.repbio.2017.10.006

Immunoglobulins from sera of patients with antiphospholipid syndrome (APS) decrease trophoblast cell invasion in vitro. This study aimed to extend understanding of cellular effects of immunoglobulins from APS (aPL+) in HTR-8/SVneo cells. aPL+ IgG induced change in effector molecules important for cell invasion was investigated further. After 1h of culture 21% cells bound aPL+ IgG, as opposed to 6% in control (aPL-). This was accompanied by increase in phospho-p38 at 30min. After 24h treatment aPL+IgG decreased protein levels of integrin subunits α1 (78% of control; p<0.01), α4 (65% of control, p<0.01), α5 (76% of control; p<0.01) and β1 (80% of control; p<0.01), and secreted gal-1 (68% of control; p<0.05). ProMMP-9 was reduced to 70% of control (p<0.001). Treatment with inhibitor of p38 MAPK signaling SB202190 reversed inhibition in integrin β1 and secreted gal-1. Involvement of p38 MAPK signaling and decrease in integrin subunit α4 proMMP-9, and secreted gal-1 in HTR-8/SVneo cells are novel and extend the list of mediators of trophoblast invasion affected by aPL.