A molecular rotor sensor for detecting mitochondrial viscosity in apoptotic cells by two-photon fluorescence lifetime imaging

Impairing mitochondrial function may change its viscosity. The detection of changes in the mitochondrial viscosity is of great significance for biomedical research. Herein, we have designed a D-π-A type two-photon fluorescent probe ((4-(bis(4-(pyridin-4-yl)phenyl)amino)styryl)-1-methylpyridin-1-ium...

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Bibliographic Details
Published inNew journal of chemistry Vol. 44; no. 26; pp. 11342 - 11348
Main Authors Hou, Ming-Xuan, Liu, Liu-Yi, Wang, Kang-Nan, Chao, Xi-Juan, Liu, Rong-Xue, Mao, Zong-Wan
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 14.07.2020
Royal Society of Chemistry
Subjects
Apoptosis
Biocompatibility
Cells (biology)
Change detection
Chemistry
Chemistry, Multidisciplinary
Fluorescent indicators
Microscopy
Mitochondria
Photons
Physical Sciences
Science & Technology
Viscosity
CYTOPLASM
VISCOMETER
MICROSCOPY
PROBE
LIVING CELLS
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Summary:Impairing mitochondrial function may change its viscosity. The detection of changes in the mitochondrial viscosity is of great significance for biomedical research. Herein, we have designed a D-π-A type two-photon fluorescent probe ((4-(bis(4-(pyridin-4-yl)phenyl)amino)styryl)-1-methylpyridin-1-ium iodide) ( DPTPA-Py ) with a triphenylamine derivative as the electron donor and pyridinium as the electron acceptor for detecting mitochondrial viscosity in living cells. It is found that both fluorescence intensity and fluorescence lifetime of DPTPA-Py are viscosity-dependent, and the probe exhibits a strong anti-interference ability in the detection of viscosity. The colocalization experiments reveal that DPTPA-Py is located in mitochondria only and is highly biocompatible. The probe shows no significant effects on the mitochondrial membrane potential. In addition, the probe is successfully used to monitor mitochondrial viscosity changes during apoptosis of living cells by two-photon microscopy (TPM) and fluorescence lifetime imaging microscopy (FLIM). A two-photon fluorescent probe was developed for detecting mitochondrial viscosity during apoptosis of living cells by two-photon microscopy (TPM) and fluorescence lifetime imaging microscopy (FLIM) with good selectivity and highly biocompatible.
Bibliography:Electronic supplementary information (ESI) available. See DOI
10.1039/d0nj02108c
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ISSN:1144-0546
1369-9261
DOI:10.1039/d0nj02108c