An integrated strategy using UPLC–QTOF-MSE and UPLC–QTOF-MRM (enhanced target) for pharmacokinetics study of wine processed Schisandra Chinensis fructus in rats

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 139; pp. 165 - 178
• Main Authors: Liu, Kuangyi, Song, Yonggui, Liu, Yali, Peng, Mi, Li, Hanyun, Li, Xueliang, Feng, Bingwei, Xu, Pengfei, Su, Dan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.05.2017
• Subjects: Herbal medicine; Pharmacokinetics; QTOF-MRM; QTOF-MSE; Wine processed Schisandra Chinensis fructus; Herbal medicine; Wine processed Schisandra Chinensis fructus; Pharmacokinetics; QTOF-MRM; QTOF-MSE
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2017.02.043

[Display omitted] •Development of an integrated strategy for extensive pharmacokinetics assessments.•Multi-components analysis based on the use of UPLC-TOF-MSE and UPLC-TOF-MRM.•Application for the simultaneous determination of 12 lignans in rat plasma with or without authentic standards.•Significant differences in pharmacokinetics parameters of lignans was observed between schizandrin and gomisin compounds. Currently the pharmacokinetic (PK) research of herbal medicines is still limited and facing critical technical challenges on quantitative analysis of multi-components from biological matrices which often accompanied by lacking of authentic standards and low concentration. This present work contributes to the development of an integrated strategy for extensive pharmacokinetics assessments, and a selective and sensitive method independent of authentic standards for multi-components analysis based on the use of ultra-performance liquid chromatography/quadrupole-time-of-flight/MSE (UPLC-TOF-MSE) and UPLC-TOF-MRM (rnhanced target). Initially, phytochemicals were identified by UPLC-TOF-MSE analysis, subsequently the identified components were matched with authentic standards and pre-classified, and UPLC–QTOF-MRM method optimized and developed. To guarantee reliable results, three rules are necessary: (1) detection with a mass error of less than 5ppm; (2) same class chemical compositions with structural high similarity between analytes with and without authentic reference substance; (3) a matching retention time between TOF-MRM mode and TOF-MSE within 0.2min. The developed and validated method was applied for the simultaneous determination of 12 lignans in rat plasma after administered with wine processed Schisandra Chinensis fructus (WPSCF) extract. Such an approach was found capable of providing extensive pharmacokinetic profiles of multi-components absorbed into blood after oral administrated with WPSCF extract. The results also indicated that significant difference in pharmacokinetics parameters of dibenzocyclooctadiene lignans was observed between schizandrin and gomisin compounds. For lignans, the absorption via gastrointestinal tract were all rapid and maintained relatively long retention time, especially for schisantherin A and schisantherin B with higher plasma exposure.