High-throughput hydrolysis of starch during permeation across α-amylase-immobilized porous hollow-fiber membranes

• Published in: Radiation physics and chemistry (Oxford, England : 1993) Vol. 63; no. 2; pp. 143 - 149
• Main Authors: Miura, Suguru, Kubota, Noboru, Kawakita, Hidetaka, Saito, Kyoichi, Sugita, Kazuyuki, Watanabe, Kohei, Sugo, Takanobu
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.02.2002
• ISSN: 0969-806X; 1879-0895
• DOI: 10.1016/S0969-806X(01)00222-5

Two kinds of supporting porous membranes, ethanolamine (EA) and phenol (Ph) fibers, for immobilization of α-amylase were prepared by radiation-induced graft polymerization of an epoxy-group-containing monomer, glycidyl methacrylate, onto a porous hollow-fiber membrane, and subsequent ring-opening with EA and Ph, respectively. An α-amylase solution was forced to permeate radially outward through the pores of the EA and Ph fibers. α-Amylase was captured at a density of 0.15 and 6.6 g/L of the membrane by the graft chain containing 2-hydroxyethylamino and phenyl groups, respectively. A permeation pressure of 0.10 MPa provided a space velocity of 780 and 1500 h −1 for the α-amylase-immobilized EA and Ph fibers, respectively. Quantitative hydrolysis of starch during permeation of a 20 g/L starch solution in the buffer across the α-amylase-immobilized Ph fiber was attained up to a space velocity of about 2000 h −1; this was achieved because of negligible diffusional mass-transfer resistance of the starch to the α-amylase due to convective flow, whereas an enzyme reaction-controlled system was observed for the α-amylase-immobilized EA fiber.