Prophylactic effects of alkaloids from Ba lotus seeds on L-NNA-induced hypertension in mice

• Published in: Chinese journal of natural medicines Vol. 14; no. 11; pp. 835 - 843
• Main Authors: SUN, Peng, ZHU, Kai, WANG, Cun, LIU, Wei-Wei, PENG, De-Guang, ZHAO, Xin
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.11.2016
• Subjects: Alkaloids; Alkaloids - administration & dosage; Animals; Ba lotus seed; Blood Pressure - drug effects; Disease Models, Animal; Humans; Hypertension; Hypertension - chemically induced; Hypertension - drug therapy; Hypertension - metabolism; Hypertension - physiopathology; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; L-NNA; Male; Mice; Mice, Inbred ICR; Nitroarginine; Nymphaeaceae - chemistry; Seeds - chemistry; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; Hypertension; Alkaloids; Mice; L-NNA; Ba lotus seed
• ISSN: 1875-5364; 1875-5364
• DOI: 10.1016/S1875-5364(16)30100-5

Alkaloids from Ba lotus seeds (ABLS) are a kind of important functional compounds in lotus seeds. The present study was designed to determine its hypertension prophylactic effects in the L-NNA-induced mouse hypertension model. The mice were treated with ABLS, the serum and tissues levels of NO, MDA, ET-1, VEGF, and CGRP were determined using the experimental kits, the mRNA levels of various genes in the heart muscle and blood vessel tissues were further determined by RT-PCR assay. ABLS could reduce the systolic blood pressure (SBP), mean blood pressure (MBP), and diastolic blood pressure (DBP), compared to that of the model control group. After ABLS treatment, the NO (nitric oxide) contents in serum, heart, liver, kidney and stomach of the mice were higher than that of the control mice, but the MDA (malonaldehyde) contents were lower than that of the control mice. The serum levels of ET-1 (endothelin-1), VEGF (vascular endothelial growth factor) were decreased after ABLS treatment, but CGRP (calcium gene related peptide) level was increased. The ABLS treated mice had higher mRNA expressions of HO-1, nNOS, and eNOS and lower expressions of ADM, RAMP2, IL-1β, TNF-α, and iNOS than the control mice. Higher concentration of ABLS had greater prophylactic effects, which were close to that of the hypertension drug captopril. These results indicated the hypertension prophylactic effects of ABLS could be further explored as novel medicine or functional food in the future.