Live Cell Electron Microscopy Using Graphene Veils

As a promising tool over the optical resolution limits, liquid electron microscopy is practically utilized to visualize the structural information on wet biological specimens, such as cells, proteins, and nucleic acids. However, the functionality of biomolecules during their observation is still con...

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Bibliographic Details
Published inNano letters Vol. 20; no. 6; pp. 4708 - 4713
Main Authors Koo, Kunmo, Dae, Kyun Seong, Hahn, Young Ki, Yuk, Jong Min
Format Journal Article
LanguageEnglish
Published United States 10.06.2020
Subjects
scanning electron microscopy
in situ electron microscopy
bacteria imaging
radiation damage
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Summary:As a promising tool over the optical resolution limits, liquid electron microscopy is practically utilized to visualize the structural information on wet biological specimens, such as cells, proteins, and nucleic acids. However, the functionality of biomolecules during their observation is still controversial. Here we show the feasibility of live-cell electron microscopy using graphene veils. We demonstrate that the electron dose resistivity of live bacterial cells increases to 100-fold in graphene veils, and thus they maintain their structures and functions after electron microscopy experiments. Our results provide the guidelines and show possibilities for the electron microscopy imaging of live cells and functional biomolecules.
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ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.0c00715