Rapid determination of 25 drug residues in aquatic products by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry

• Published in: Sepu Vol. 36; no. 2; p. 114
• Main Authors: Zhang, Xianchen, Li, Rong, Zhang, Pengjie, Wu, Xia, Hua, Hongbo, Yang, Luqi, Lu, Junwen, Rong, Yutang
• Format: Journal Article
• Language: Chinese
• Published: China 08.02.2018
• Subjects: drug residues; quadrupole/electrostatic field orbitrap high resolution mass spectrometry (Q Orbitrap HRMS); aquatic products; enhanced matrix removal of lipids (EMR-Lipid); ultra performance liquid chromatography (UPLC); QuEChERS
• ISSN: 1000-8713
• DOI: 10.3724/SP.J.1123.2017.10010

An ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q Orbitrap HRMS) method was developed for the rapid screening of 25 drug residues in aquatic products by the application of a novel enhanced matrix removal of lipids (EMR-Lipid). The samples were extracted with acetonitrile, cleaned-up with EMR-Lipid, and salted out with 3.0 g sodium chloride and 3.0 g sodium sulfate. The separation of the 25 drug residues was performed on an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) with gradient elution. Acetonitrile containing 0.1% (v/v) formic acid and 0.1% (v/v) formic acid aqueous solution were used as mobile phases. The compounds were detected by Full scan/date dependent MS/MS (Full MS/dd-MS ) via heated electrospray ionization (HESI) source. The calibration curves were linear with correlation coefficients ( ) greater than 0.997. The limits of detection (LODs) ranged from 0.1 μg/kg to 1.0 μg/kg. The average spiked recoveries of the 25 target compounds were between 70.1% and 108.9% with relative standard derivations (RSDs) from 2.1% to 13.8%. Compared with the previous methods, this method has characteristics of simpler sample preparation and higher sensitivity.