Determination of bovine serum albumin by capillary zone electrophoresis with end-column amperometric detection at the carbon fiber microdisk array electrode

Capillary zone electrophoresis (CZE) was employed to the analysis of bovine serum albumin (BSA) using an end-column amperometric detector with a carbon fiber microdisk array electrode. The optimum conditions of separation and detection are 0.025 mol l −1 KH 2PO 4-0.02 mol l −1 NaOH for the buffer so...

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Bibliographic Details
Published inAnalytica chimica acta Vol. 342; no. 1; pp. 67 - 74
Main Authors Jin, Wenrui, Weng, Qianfeng, Wu, Jianren
Format Journal Article
LanguageEnglish
Published Elsevier B.V 21.04.1997
Subjects
Amperometry
Bovine serum albumin
Electrophoresis
Serum
Amperometry
Bovine serum albumin
Electrophoresis
Serum
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Summary:Capillary zone electrophoresis (CZE) was employed to the analysis of bovine serum albumin (BSA) using an end-column amperometric detector with a carbon fiber microdisk array electrode. The optimum conditions of separation and detection are 0.025 mol l −1 KH 2PO 4-0.02 mol l −1 NaOH for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, and 0.90 V vs. SCE for the detection potential. The limit of detection is 4 × 10 −7 mol l −1 or 0.7 fmol ( S N = 2 ). The method was applied to determining BSA in bovine serum.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(96)00530-2