Ursolic acid alleviates meiotic abnormalities induced by 3-nitropropionic acid in mouse oocytes

• Published in: Toxicology and applied pharmacology Vol. 485; p. 116910
• Main Authors: Han, Tiancang, Sun, Zhaoyang, Zhang, Hongbo, Zhao, Yuhan, Jiao, Anhui, Gao, Qingshan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2024
• Subjects: 3-nitropropionic acid; Meiotic division; Oocytes; Oxidative stress; Ursolic acid; Oxidative stress; Ursolic acid; 3-nitropropionic acid; Meiotic division; Oocytes
• ISSN: 0041-008X; 1096-0333
• DOI: 10.1016/j.taap.2024.116910

3-nitropropionic acid (3-NPA), a toxic metabolite produced by mold, is mainly found in moldy sugarcane. 3-NPA inhibits the activity of succinate dehydrogenase that can induce oxidative stress injury in cells, reduce ATP production and induce oxidative stress in mouse ovaries to cause reproductive disorders. Ursolic acid (UA) has a variety of biological activities and is a pentacyclic triterpene compound found in many plants. This experiment aimed to investigate the cytotoxicity of 3-NPA during mouse oocyte in vitro maturation and the protective effects of UA on oocytes challenged with 3-NPA. The results showed that UA could alleviate 3-NPA-induced oocyte meiotic maturation failure. Specifically, 3-NPA induced a decrease in the first polar body extrusion rate of oocytes, abnormal distribution of cortical granules, and an increase in the proportion of spindle abnormalities. In addition, 3-NPA caused mitochondrial dysfunction and induced oxidative stress, including decreases in the GSH, mitochondrial membrane potential and ATP levels, and increases in the ROS levels, and these effects led to apoptosis and autophagy. The addition of UA could significantly improve the adverse effects caused by 3-NPA. In general, our data show that 3-NPA affects the normal development of oocytes during the in vitro culture, and the addition of UA can effectively repair the damage caused by 3-NPA to oocytes. [Display omitted] •3-nitropropionic acid induces oxidative stress in oocytes.•3-nitropropionic acid inhibits nuclear and cytoplasmic maturation of oocytes.•3-nitropropionic acid induces autophagy and apoptosis in oocytes.•Ursolic acid alleviates oocytes quality decline caused by 3-nitropropionic acid.