Inhibition of Wnt11 impairs the osteogenesis and aggravates the inflammatory response of human mesenchymal stem cells under LPS-induced inflammatory condition

• Published in: Biochemical and biophysical research communications Vol. 661; pp. 82 - 88
• Main Authors: Ding, Gao, Mu-Guo, Song
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18.06.2023
• Subjects: Animals; Cell Differentiation; Cells, Cultured; Human mesenchymal stem cells; Humans; Immunologic Factors - pharmacology; Inflammation - metabolism; Inflammatory response; Lipopolysaccharide; Lipopolysaccharides - pharmacology; Mesenchymal Stem Cells; Osteogenesis; Osteogenic differentiation; Wnt Proteins - metabolism; Wnt11; Wnt11; hMSCs; Runx-2; OC; ALP; Lipopolysaccharide; Inflammatory response; Human mesenchymal stem cells; Osteogenic differentiation; LPS; ELISA
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2023.04.027

In infectious bone defect, osteogenesis is very particularly important for treating. Currently, mesenchymal stem cells (MSCs) become a promising treatment protocol in clinical practice. In infectious environment, lipopolysaccharide (LPS) not only affects the osteogenic differentiation of MSCs, but also incurs inflammatory reaction from the host or cells and prompts the secretion of inflammatory cytokines. Wnt11 plays an important role of enhancing osteogenic ability of MSCs in treating bone infectious animal model in vivo. However, whether Wnt11 enhances the osteogenic capacity or influences the inflammatory reaction under inflammatory condition mediated by LPS in vitro remains unknown. In this study, we investigated the role of Wnt11 on the osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) and the effect on the inflammatory reaction induced by LPS. Effects of Wnt11 on the osteogenic capacity of BM-MSCs and on the inhibition of inflammatory reaction induced by LPS were evaluated by Wnt11 RNAi assay, Alizarin staining, quantitative RT-PCR test, ALP activity test and ELISA assays. The results showed inhibiting Wnt11 expression exacerbated the expression of osteogenic differentiation related genes and decreased the mineral deposits formation. Moreover, inhibiting Wnt11 expression also exacerbated the inflammatory factors release, indicating Wnt11 might play an important role of enhancing the osteogenic differentiation of BM-MSCs and inhibiting the inflammatory reaction induced by LPS. •It is the first to study the role of wnt11 gene on osteogenic differentiation of hMSCs under LPS induced inflammatory condition.•Our study manifested inhibiting wnt11 impairs the osteogenesis of hMCSs under LPS-induced inflammatory.•Our study manifested inhibiting wnt11 aggravates the inflammatory response of hMCSs under LPS-induced inflammatory.