Determination of trace fluoroquinolones in honey and milk based on cyclodextrin modified magnetic metal-organic frameworks solid phase extraction coupled with ultra-high performance liquid chromatography

• Published in: Journal of Chromatography A Vol. 1713; p. 464521
• Main Authors: Sun, Yangkun, Kuang, Jingjing, Cheng, Yongzhe, Lin, Chuhui, Zhang, Hongyang, Zhang, Min, Ning, Fanghong, Hu, Ping
• Format: Journal Article
• Language: English
• Published: Netherlands 04.01.2024
• Subjects: Animals; Chromatography, High Pressure Liquid - methods; Cyclodextrins; Fluoroquinolones - analysis; Honey - analysis; Humans; Magnetic Phenomena; Metal-Organic Frameworks - chemistry; Milk - chemistry; Solid Phase Extraction - methods; FeO@UiO-66-CD; Fluoroquinolone; Magnetic solid phase extraction; Ultra-high performance liquid chromatography
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2023.464521

Long-term intake of animal-derived foods with excessive fluoroquinolones (FQs) will cause damage to human health, so it is critical to establish a feasible approach for sensitive and rapid monitoring of FQs residues. In this study, a new cyclodextrin modified magnetic metal-organic frameworks (Fe O @UiO-66-CD) was successfully synthesized by amidation reaction and applied to magnetic solid phase extraction (MSPE) for FQs analysis. The adsorption behavior of Fe O @UiO-66-CD was consistent with the pseudo-second-order kinetics and Freundlich isothermal adsorption model, which indicated that the designed material had various interactions on FQs, such as host-guest interaction and π-π interaction. The parameters of MSPE were optimized and the determination method of norfloxacin, enrofloxacin, lomefloxacin and gatifloxacin was established by using MSPE combined with ultra-high performance liquid chromatography (UHPLC) and fluorescence detector (FLD). The method validation results displayed that the detection limits were 0.02-0.09 ng/mL, and the RSDs of intra-day and inter-day precision were less than 4.1 and 6.4 %, respectively. In the target FQs analysis of real honey and milk samples, the recoveries at different fortified concentrations were in the ranges of 88.4 % to 108.6 % with RSD ≤ 5.7 %. The results showed that the proposed method was sensitive, accurate and reliable for the determination of trace FQs in animal-derived foods.