TEAD3 inhibits the proliferation and metastasis of prostate cancer via suppressing ADRBK2

• Published in: Biochemical and biophysical research communications Vol. 654; pp. 120 - 127
• Main Authors: Wang, Chunhui, Chen, Songmao, Li, Xiaoli, Fan, Lin, Zhou, Zhe, Zhang, Mingpeng, Shao, Yi, Shang, Zhiqun, Niu, Yuanjie
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.04.2023
• Subjects: ADRBK2; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation; Gene Expression Regulation, Neoplastic; Hedgehog Proteins - metabolism; Hedgehog signaling pathway; Humans; Male; Prostate cancer; Prostatic Hyperplasia - genetics; Prostatic Hyperplasia - metabolism; Prostatic Neoplasms - pathology; TEA Domain Transcription Factors; TEAD3; Hedgehog signaling pathway; ADRBK2; Prostate cancer; TEAD3
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2023.02.081

TEAD3 acts as a transcription factor in many tumors to promote tumor occurrence and development. But in prostate cancer (PCa), it appears as a tumor suppressor gene. Recent studies have shown that this may be related to subcellular localization and posttranslational modification. We found that TEAD3 was down-expressed in PCa. Immunohistochemistry of clinical PCa specimens confirmed that TEAD3 expression was the highest in benign prostatic hyperplasia (BPH) tissues, followed by primary PCa tissues, and the lowest in metastatic PCa tissues, and its expression level was positively correlated with overall survival. MTT assay, clone formation assay, and scratch assay confirmed that overexpression of TEAD3 could significantly inhibit the proliferation and migration of PCa cells. Next-generation sequencing results indicated that Hedgehog (Hh) signaling pathway was significantly inhibited after overexpression of TEAD3. Rescue assays suggested that ADRBK2 could reverse the proliferation and migration ability caused by overexpression of TEAD3. TEAD3 is downregulated in PCa and associated with poor patient prognosis. Overexpression of TEAD3 inhibits the proliferation and migration ability of PCa cells via restraining the mRNA level of ADRBK2. These results indicate that TEAD3 was down-expressed in PCa patients and was positively correlated with a high Gleason score and poor prognosis. Mechanistically, we found that the upregulation of TEAD3 inhibits the proliferation and metastasis of prostate cancer by inhibiting the expression of ADRBK2. •TEAD3 is down-expressed in prostate cancer and is associated with a worse prognosis.•Overexpression of TEAD3 can significantly inhibit the proliferation and metastasis of prostate cancer cells.•TEAD3 regulates the Hedgehog signaling pathway in prostate cancer.•Overexpression of TEAD3 inhibits prostate cancer proliferation and migration by restraining ADRBK2 expression.•Overexpression of TEAD3 significantly inhibits prostate cancer tumor growth in vivo.