Biosynthesis, characterization, magnetic hyperthermia, and in vitro toxicity evaluation of quercetin-loaded magnetoliposome lipid bilayer hybrid system on MCF-7 breast cancer

• Published in: Biochimica et biophysica acta. General subjects Vol. 1868; no. 3; p. 130543
• Main Authors: Elbeltagi, Shehab, Saeedi, Ahmad M., Eldin, Zienab E., Alfassam, Haifa E., Alharbi, Hanan M., Madkhali, Nawal, Shakor, Abo Bakr Abdel, El-Aal, Mohamed Abd
• Format: Journal Article
• Language: English
• Published: Netherlands 01.03.2024
• Subjects: Breast Neoplasms - drug therapy; Female; Humans; Hyperthermia, Induced - methods; Lipid Bilayers; Liposomes - chemistry; Magnetic Phenomena; MCF-7 Cells; Quercetin - chemistry; Quercetin - pharmacology; Spectroscopy, Fourier Transform Infrared; And MCF-7 breast cancer; MHT; MgFeO; Chemotherapy/hyperthermia; Liposomes; Quercetin
• ISSN: 0304-4165; 1872-8006; 1872-8006
• DOI: 10.1016/j.bbagen.2023.130543

Novel biocompatible and effective hyperthermia (HT) treatment materials for breast cancer therapeutic have recently attracting researchers, because of their effective ablation of cancer cells and negligible damage to healthy cells. Magnetoliposome (MLs) have numerous possibilities for utilize in cancer treatment, including smart drug delivery (SDD) mediated through alternating magnetic fields (AMF). In this work, magnesium ferrite (MgFe O ) encapsulated with liposomes lipid bilayer (MLs), Quercetin (Q)-loaded MgFe O @Liposomes (Q-MLs) nano-hybrid system were successfully synthesized for magnetic hyperthermia (MHT) and SDD applications. The hybrid system was well-investigated by different techniques using X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR), Energy dispersive X-ray (EDX), Vibrating sample magnetometer (VSM), Transmission electron microscope (TEM), and Zeta Potential (ZP). The characterization results confirmed the improving quercetin-loading on the MLs surface. TEM analysis indicated the synthesized MgFe O , MLs, and Q-MLs were spherical with an average size of 23.7, 35.5, and 329.5 nm, respectively. The VSM results revealed that the MgFe O exhibit excellent and effective saturation magnetization (M ) (40.5 emu/g). Quercetin drug loading and entrapment efficiency were found to be equal to 2.1 ± 0.1% and 42.3 ± 2.2%, respectively. The in-vitro Q release from Q-loaded MLs was found 40.2% at pH 5.1 and 69.87% at pH 7.4, verifying the Q-loading pH sensitivity. The MLs and Q-MLs hybrid system as MHT agents exhibit specific absorption rate (SAR) values of 197 and 205 W/g, correspondingly. Furthermore, the Q-MLs cytotoxicity was studied on the MCF-7 breast cancer cell line, and the obtained data demonstrated that the Q-MLs have a high cytotoxicity effect compared to MLs and free Q.