Cell wall assembly in vitro from Chlamydomonas reinhardi

The conditions for in vitro dissociation and reassembly of the cell wall of Chlamydomonas reinhardi have been investigated. The cell wall dissociates in aqueous 8 M lithium chloride to two homogeneous components (6.8 S20w and 9.3 S20w)and dialysis of the cell wall subunits against water causes reass...

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Bibliographic Details
Published inPlanta Vol. 115; no. 1; p. 17
Main Author Hills, G J
Format Journal Article
LanguageEnglish
Published Germany 01.03.1973
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Summary:The conditions for in vitro dissociation and reassembly of the cell wall of Chlamydomonas reinhardi have been investigated. The cell wall dissociates in aqueous 8 M lithium chloride to two homogeneous components (6.8 S20w and 9.3 S20w)and dialysis of the cell wall subunits against water causes reassembly of a product having the same overall morphology and chemically similar to the original cell walls. Cell wall subunits in 8 M lithium chloride alone do not reassemble on dialysis against water to form cell walls but a nucleation centre has to be provided.
ISSN:0032-0935
DOI:10.1007/BF00388601