Adenovirus-specific T-cell Subsets in Human Peripheral Blood and After IFN-γ Immunomagnetic Selection

• Published in: Journal of immunotherapy (1997) Vol. 39; no. 1; p. 27
• Main Authors: Qian, Chongsheng, Wang, Yingying, Cai, Huili, Laroye, Caroline, De Carvalho Bittencourt, Marcelo, Clement, Laurence, Stoltz, Jean-François, Decot, Véronique, Reppel, Loïc, Bensoussan, Danièle
• Format: Journal Article
• Language: English
• Published: United States 01.01.2016
• Subjects: Adenoviridae - immunology; Adenoviridae Infections - immunology; Adenoviridae Infections - therapy; Antigens, Surface - metabolism; Cell Culture Techniques; Cytotoxicity, Immunologic; Healthy Volunteers; Humans; Immunologic Memory; Immunomagnetic Separation; Immunophenotyping; Immunotherapy, Adoptive; Interferon-gamma - metabolism; Lymphocyte Count; Phenotype; T-Cell Antigen Receptor Specificity - immunology; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - metabolism
• ISSN: 1537-4513
• DOI: 10.1097/CJI.0000000000000105

Adoptive antiviral cellular immunotherapy by infusion of virus-specific T cells (VSTs) is becoming an alternative treatment for viral infection after hematopoietic stem cell transplantation. The T memory stem cell (TSCM) subset was recently described as exhibiting self-renewal and multipotency properties which are required for sustained efficacy in vivo. We wondered if such a crucial subset for immunotherapy was present in VSTs. We identified, by flow cytometry, TSCM in adenovirus (ADV)-specific interferon (IFN)-γ+ T cells before and after IFN-γ-based immunomagnetic selection, and analyzed the distribution of the main T-cell subsets in VSTs: naive T cells (TN), TSCM, T central memory cells (TCM), T effector memory cell (TEM), and effector T cells (TEFF). In this study all of the different T-cell subsets were observed in the blood sample from healthy donor ADV-VSTs, both before and after IFN-γ-based immunomagnetic selection. As the IFN-γ-based immunomagnetic selection system sorts mainly the most differentiated T-cell subsets, we observed that TEM was always the major T-cell subset of ADV-specific T cells after immunomagnetic isolation and especially after expansion in vitro. Comparing T-cell subpopulation profiles before and after in vitro expansion, we observed that in vitro cell culture with interleukin-2 resulted in a significant expansion of TN-like, TCM, TEM, and TEFF subsets in CD4IFN-γ T cells and of TCM and TEM subsets only in CD8IFN-γ T cells. We demonstrated the presence of all T-cell subsets in IFN-γ VSTs including the TSCM subpopulation, although this was weakly selected by the IFN-γ-based immunomagnetic selection system.