Comparison of electrochemical and enzymic oxidation of 1,3-dimethyluric acid

Electrochemical oxidation of 1,3-dimethyluric acid has been studied in phosphate buffers of pH 2.3–10.3 at pyrolytic graphite electrode. The conjugate base has been found as the electroactive species and a single well-defined oxidation peak was observed. The presence of methyl groups have been found...

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Bibliographic Details
Published inBioelectrochemistry and Bioenergetics Vol. 44; no. 2; pp. 201 - 208
Main Authors Goyal, Rajendra N., Singhal, Naveen K.
Format Journal Article
LanguageEnglish
Published Elsevier S.A 1998
Subjects
1,3-dimethyluric acid
Electrooxidation
Enzymic oxidation
Electrooxidation
Enzymic oxidation
1,3-dimethyluric acid
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Summary:Electrochemical oxidation of 1,3-dimethyluric acid has been studied in phosphate buffers of pH 2.3–10.3 at pyrolytic graphite electrode. The conjugate base has been found as the electroactive species and a single well-defined oxidation peak was observed. The presence of methyl groups have been found to shift E p of uric acid towards less positive potential by 30–40 mV. The products of electrooxidation were separated by HPLC at pH 3.0 and were characterized as 1,3-dimethylalloxan and urea. At pH 7.0, the major product was 5-hydroxyhydantoin-5-( N-methyl) carboxamide. The spectral studies during enzymic oxidation of 1,3-dimethyluric acid generated UV-absorbing intermediate similar to electrooxidation and the rate of decay was found to be same in both the cases. The electrochemical and enzymic oxidations appear to proceed by an identical mechanism.
ISSN:0302-4598
DOI:10.1016/S0302-4598(97)00083-4