High-performance anion-exchange chromatography of sugar and glycerol phosphates on quaternary ammonium resins

Twenty-six sugar and glycerol phosphates were separated by high-performance anion-exchange chromatography on an alkyl quaternary ammonium column at pH 6.0 with 0.1 M sodium acetate eluant. Capacity, asymmetry, and response factors of the compounds vary widely. Secondary acidic dissociation constants...

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Bibliographic Details
Published inCarbohydrate research Vol. 322; no. 1; pp. 128 - 134
Main Authors Schneider, Ian C, Rhamy, Preston J, Fink-Winter, Ruth J, Reilly, Peter J
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 23.11.1999
Subjects
Dissociation constants
Glycerol phosphates
HPAEC
HPLC
Quaternary ammonium resins
Strong-base resins
Sugar phosphates
Glycerol phosphates
Strong-base resins
Quaternary ammonium resins
Sugar phosphates
Dissociation constants
HPAEC
HPLC
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Summary:Twenty-six sugar and glycerol phosphates were separated by high-performance anion-exchange chromatography on an alkyl quaternary ammonium column at pH 6.0 with 0.1 M sodium acetate eluant. Capacity, asymmetry, and response factors of the compounds vary widely. Secondary acidic dissociation constants of 14 of these phosphates were determined in an attempt to explain the separation mechanism.
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(99)00210-4