Competitive immunoassay (Cat-EIA), a helpful technique for catalytic antibody detection. Part I

A competitive immunoassay procedure for the screening of catalytic antibodies is reported. This screening approach (Cat-EIA) is a modification of the well-known Cat-ELISA technique avoiding the substrate modification step. It has been developed for a bimolecular reaction and has been tested on a hig...

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Bibliographic Details
Published inTetrahedron letters Vol. 40; no. 10; pp. 1887 - 1890
Main Authors Taran, F., Renard, P.Y., Créminon, C., Valleix, A., Frobert, Y., Pradelles, P., Grassi, J., Mioskowski, C.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 05.03.1999
Subjects
Antibodies
Catalysis
Phenol
Antibodies
Phenol
Catalysis
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Summary:A competitive immunoassay procedure for the screening of catalytic antibodies is reported. This screening approach (Cat-EIA) is a modification of the well-known Cat-ELISA technique avoiding the substrate modification step. It has been developed for a bimolecular reaction and has been tested on a high number of hybridoma clones. The current study explores for the first time the utility and feasibility of this method for the early detection of catalytic antibodies. A new procedure for the early detection of catalytic antibodies was evaluated on a bimolecular reaction.
ISSN:0040-4039
1873-3581
DOI:10.1016/S0040-4039(99)00067-2