Anti-oxidant, DNA-damage protection and anti-cancer properties of n-butanol extract of the endemic Perralderia coronopifolia

• Published in: Bangladesh Journal of Pharmacology Vol. 13; no. 1
• Main Authors: Khadidja Bekhouche, Tevfik Ozen, Sara Boussaha, Serkan Koldas, Semiha Yenigun, Somia Lassed, Ibrahim Demirtas, Fadila Benayache, Samir Benayache, Djamila Zama
• Format: Journal Article
• Language: English
• Published: Bangladesh Pharmacological Society 15.03.2018
• Subjects: anticancer activity; antioxidant activity; flavonoids; oxidative DNA-damage inhibition; Perralderia coronopifolia Coss; Phenolic content
• ISSN: 1991-0088
• DOI: 10.3329/bjp.v13i1.34255

This study was designed to evaluate in vitro the total phenolic, flavonoid content, anti-oxidant activity, oxidative DNA-damage protection and anti-cancer activity of n-butanol extract from Perralderia coronopifolia, endemic plant. DNA protection capacity was performed using 46966 plasmid DNA against UV-photolysis of H2O2-induced oxidative damage. The anti-prolifer-ative effects of extract were performed on HeLa and C6 cells. Experimental results showed that extract had convenient number of phenolic and flavonoids. Furthermore, it provided strong anti-oxidant, reducing power, hydrogen peroxide and higher DPPH· scavenging ability with IC50= 7.02 ± 0.02 µg/mL. As it shown an oxidative plasmid DNA-damage inhibition against UV-photolysis of H2O2, an anti-cancer activity athigher concentrations in cells. The data obtained that P. coronopifolia extract could be useful in human protection against infection and degenerative illness. Video Component of Methodology: Metal chelating activity:   2 min 56 sec   Full Screen   Alternate