Zaleya decandra exerts anti-cancer activity in HCT-116 cancer cells by impairing mitochondrial activity

An attempt has been made to investigate the role of Zaleya decandra on the survival of HCT colon cancer cells in vitro. The cell viability and apoptosis were assessed using MTT assay and acridine orange/ethidium bromide staining, respectively. Mitrochondrial membrane permeability was assessed using...

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Bibliographic Details
Published inBangladesh Journal of Pharmacology Vol. 14; no. 4
Main Authors Malarvizhi Deivasigamani, Ajay Kasivishwanathan Chandrasekar, Sridhar Muthusami, Gopalakrishnan Velliyur Kanniappan, Hariprasath Lakshmanan
Format Journal Article
LanguageEnglish
Published Bangladesh Pharmacological Society 01.01.2019
Subjects
Apoptosis
Caspases
Colon cancer cell
MTT
ROS
Zaleya decandra
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Summary:An attempt has been made to investigate the role of Zaleya decandra on the survival of HCT colon cancer cells in vitro. The cell viability and apoptosis were assessed using MTT assay and acridine orange/ethidium bromide staining, respectively. Mitrochondrial membrane permeability was assessed using rhodamine-123 staining. The activities of caspases were evaluated using ELISA kits. Treatment with Z. decandra extract increased the production of ROS in HCT cells and impaired the mitochondrial activity as evidenced by decreased reduction of tetrazolium salt and enhanced mitochondrial membrane permeability. The onset of apoptosis was evident in cells treated with the extact in acridine orange/ethidium bromide staining and was associated with elevated levels of initiator and effector caspases in the conditioned medium. In conclusion, Z. decandra treatment promoted the apoptosis of HCT colon cancer cells. Video Clip of Methodology: 6 min 53 sec:  Full Screen   Alternate
ISSN:1991-0088
DOI:10.3329/bjp.v14i4.42098