Crystal structure of the GH-46 subclass III chitosanase from Bacillus circulans MH-K1 in complex with chitotetraose

• Published in: Biochimica et biophysica acta. General subjects Vol. 1868; no. 3; p. 130549
• Main Authors: Suzuki, Michihiko, Saito, Akihiro, Kobayashi, Mariko, Yokoyama, Tomofumi, Omiya, Shoko, Li, Jian, Sugita, Kei, Miki, Kunio, Saito, Jun-ichi, Ando, Akikazu
• Format: Journal Article
• Language: English
• Published: Netherlands 01.03.2024
• Subjects: Bacillus; Chitosan; Glucosamine - metabolism; Glycoside Hydrolases - metabolism; Oligosaccharides; Bacillus circulans; Cleavage specificity; Glycoside hydrolase; Chitosanase; GH-46

Chitosanases (EC 3.2.1.132) hydrolyze chitosan which is a polymer of glucosamine (GlcN) linked by β - 1,4 bonds, and show cleavage specificity against partially acetylated chitosan containing N-acetylglucosamine (GlcNAc) residues. Chitosanases' structural underpinnings for cleavage specificity...