The impact of different levels of functional oil supplementation in combination with salinomycin on growth performance and intestinal microbiota of broilers undergoing Eimeria challenge: An analysis of dynamics

• Published in: Research in veterinary science Vol. 172; p. 105249
• Main Authors: Stefanello, Thaís Bastos, Cardinal, Kátia Maria, Orso, Catiane, Franceschi, Carolina Haubert, Silva, Jéssica Pereira, Mann, Micheli Bertoni, Frazzon, Jeverzon, Moraes, Priscila Oliveira, Ribeiro, Andréa Machado Leal
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2024
• Subjects: 16S rRNA sequencing; Animal Feed - analysis; Animals; Broiler; Chickens - growth & development; Coccidiosis; Coccidiosis - drug therapy; Coccidiosis - parasitology; Coccidiosis - veterinary; Coccidiostats - administration & dosage; Coccidiostats - pharmacology; Diet - veterinary; Dietary Supplements; Eimeria; Eimeria - drug effects; Gastrointestinal Microbiome - drug effects; Ionophores - administration & dosage; Ionophores - pharmacology; Male; Microbiome; Polyether Polyketides; Poultry Diseases - drug therapy; Poultry Diseases - microbiology; Poultry Diseases - parasitology; Pyrans - administration & dosage; Pyrans - pharmacology; Random Allocation; Eimeria; Coccidiosis; 16S rRNA sequencing; Broiler; Microbiome
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2024.105249

The effect of salinomycin sodium alone and in combination with functional oils on performance and microbiota of broiler infected Eimeria were evaluated. 512 broilers were randomly assigned to 4 treatments (8 replicates, 16 birds/pen): a Control group (any additives); Ionophore group: salinomycin supplementation at 66 ppm (SS66); Ionophore +0.075% Functional oil (FO) group (SS66 + FO supplementation at 750 ppm); and Ionophore +0.10% FO group (SS66 + FO supplementation at 1000 ppm). At 14 days of age, birds were gavaged with 1 mL of a saline solution containing sporulated oocysts of E. tenella, E. acervulina and E. maxima. Performance indices were measured weekly. At 28 days, intestinal content was collected for microbiota analysis. Broilers of Control group presented the worst performance indices. Broilers of Ionophore + FO (0.075% and 0.10%) groups exhibited a higher BW at 28 days of age. The supplementation of Ionophore +0.075% FO resulted in a higher relative proportion of Firmicutes and a lower proportion of Actinobacteria in the ileum-jejunum. Lactobacillaceae was the dominant family in the jejunal, and ileal microbiotas of broilers fed diets supplemented with Ionophore, Ionophore +0.075% FO and Ionophore +0.10% FO. The supplementation of ionophore yielded higher numbers of Lactobacillaceae, Enterobactereaceae and Cloritridiaceae in the cecal. Ionophore associated with FO controlled the Lactobacillaceae, Enterobactereaceae and Cloritridiaceae families present in the cecum. Therefore, the combination of salinomycin with functional oil showed synergistic effect on performance and modulation of intestinal microbiota of broilers challenged with Eimeria. •Ionophore plus functional oil increased Firmicutes, decreased Actinobacteria in ileum-jejunum.•Ionophore plus functional oil controlled cecum families: Reduced Lactobacillaceae, Enterobactereaceae, Cloritridiaceae; increased Ruminococcaceae, Lachnospiraceae.•Salinomycin plus functional oil synergistic effect: Improved performance, modulated gut microbiota during Eimeria challenge.•Combination of salinomycin and essential oil can minimize the impact of coccidiosis