Konjac Glucomannan Oligosaccharides (KGMOS) Confers Innate Immunity against Phytophthora nicotianae in Tobacco

• Published in: Agriculture (Basel) Vol. 14; no. 8; p. 1289
• Main Authors: Rajib, Md Mijanur Rahman, Li, Kuikui, Bhuiyan, Md Saikat Hossain, Wang, Wenxia, Gao, Jin, Yin, Heng
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.08.2024
• Subjects: Accumulation; Antimicrobial agents; Bacteria; Catabolism; Cellular stress response; Defense industry; Disease resistance; Ecosystems; Ethylene; Evolution; Gene regulation; Genes; Hydrogen peroxide; Immune system; Innate immunity; Inoculation; Jasmonic acid; KGMOS; Late blight; Metabolites; nicotiana benthamiana; Oligosaccharides; Oxidative stress; Pathogenesis; Pathogens; Phytophthora nicotianae; Plant diseases; Plant immunity; Plant resistance; Plants; Post-harvest decay; Proteins; Salicylic acid; Seeds; Sustainable agriculture; Tobacco; transcriptome; Transcriptomics; China
• ISSN: 2077-0472; 2077-0472
• DOI: 10.3390/agriculture14081289

In this study, KGMOS (DP, 2-13), derived from KGM (Konjac glucomannan), was applied to elucidate plant immunity in a Nicotiana benthamiana Phytophthora nicotianae model. Application of KGMOS (25–100 mg/L) notably inhibited P. nicotianae, resulting in reduced disease indices and a significant accumulation of defense molecules such as H2O2 and callose. Transcriptomic analysis revealed that genes shared between KGMOS-treated and control plants are involved in signaling pathways, transcription regulation, hydrogen peroxide catabolism, and oxidative stress response. This suggests that KGMOS triggers H2O2 accumulation, callose deposition, and activation of the salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) pathways after pathogen inoculation. Upregulated defense-response genes in the KGMOS group included SA-related late blight-resistant, pathogenesis-related (PR), and JA/ET-related ethylene response factor (ERF) genes. Heatmap analysis showed more upregulated defense genes (PR and NPR) related to SA in the KGMOS-treated group than in controls. RT-qPCR validation revealed significant upregulation of SA and JA/ET pathway genes in KGMOS-treated plants. Higher SA content in these plants suggests enhanced disease resistance. This study concludes that KGMOS pre-treatment induced resistance against P. nicotianae, especially at a lower concentration (25 mg/L). These findings could offer valuable insights for the future application of KGMOS in controlling plant diseases for sustainable agriculture and postharvest management.