Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata

• Published in: Food & function Vol. 11; no. 8; pp. 7104 - 7114
• Main Authors: Qian, Kai, Tan, Ting, Ouyang, Hui, Yang, Shi-Lin, Zhu, Wei-Feng, Liu, Rong-Hua, Wen, Quan, Feng, Yu-Lin
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 01.08.2020
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Cellulose; Chemical analysis; Chromatography, Gel; Diabetes mellitus; Diet; Dietary Supplements; Down-Regulation - drug effects; FOXO1 protein; Gel chromatography; Glucan; Glucans - chemistry; Glucose; Glucose - analysis; Hep G2 Cells; Humans; Hypoglycemic Agents - pharmacology; Insulin; Insulin Resistance - physiology; Molecular weight; Monosaccharides; Monosaccharides - analysis; Palmitic acid; Plant Roots - chemistry; Polysaccharides - chemistry; Polysaccharides - pharmacology; Pueraria - chemistry; Pueraria lobata; Roots; Structural analysis; Up-Regulation - drug effects
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d0fo01234c

A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification. The average molecular weight of PLP-1 was 16.2 kDa. Monosaccharide composition analysis showed that PLP-1 was composed of glucose as a glucan. The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0. Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells. The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells. Therefore, PLP-1 could serve as a dietary supplement to ameliorate insulin resistance for diabetic patients.