In vivo effect of diosmin on carrageenan and CCl4-induced lipid peroxidation in rat liver microsomes

• Published in: Journal of biochemical toxicology Vol. 11; no. 1; p. 27
• Main Authors: Melin, A M, Perromat, A, Clerc, M
• Format: Journal Article
• Language: English
• Published: United States 1996
• Subjects: Animals; Antioxidants - pharmacology; Carbon Tetrachloride - administration & dosage; Carbon Tetrachloride - toxicity; Carrageenan - administration & dosage; Carrageenan - toxicity; Chromatography, High Pressure Liquid; Diosmin - administration & dosage; Diosmin - pharmacology; Fatty Acids, Unsaturated - analysis; Fatty Acids, Unsaturated - metabolism; Injections, Intraperitoneal; Lipid Peroxidation - drug effects; Male; Microsomes, Liver - drug effects; Microsomes, Liver - metabolism; Rats; Rats, Wistar; Software; Thiobarbituric Acid Reactive Substances - analysis; Thiobarbituric Acid Reactive Substances - metabolism; Vitamin A - analysis; Vitamin A - metabolism
• ISSN: 0887-2082
• DOI: 10.1002/(SICI)1522-7146(1996)11:1<27::AID-JBT4>3.0.CO;2-X

The aim of this study was to compare the protective effect of a flavonoid, the 3',5,7-trihydroxy-4'-methoxyflavone 7-rutinoside or diosmin, on liver microsomal lipid peroxidation induced in rats by either carbon tetrachloride or carrageenan. Thirty rats were divided into five groups. Group 1 received no chemical product and was considered as control. Groups 2 and 3 received either an intraperitoneal injection of carrageenan or carbon tetrachloride 48 or 24 hours before killing, respectively. Groups 4 and 5 were treated first with an intraperitoneal injection of diosmin and then by carrageenan (group 4) or carbon tetrachloride (group 5) 48 or 24 hours before killing, respectively. The lipoperoxidant effect of carrageenan and carbon tetrachloride was demonstrated by both significant decreases in polyunsaturated fatty acids, principally 20:4 (n - 6) (p < 0.05) and of vitamin A (p < 0.05) in groups 2 and 3. With diosmin treatment, only thiobarbituric acid reactive substances significantly decreased in group 4, whereas vitamin A level increased. These results could suggest that the effect of diosmin differs with the choice of chemical product used; it seems a better antioxidant against products inducing inflammation.