Assessment of Puccinia polliniicola as a potential biological control agent for Microstegium vimineum

• Published in: Pest management science Vol. 80; no. 9; pp. 4637 - 4649
• Main Authors: Tan, Min, Fang, Yanxia, Zhang, Yuanhe, Qiang, Sheng
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.09.2024; Wiley Subscription Services, Inc
• Subjects: Biological control; Biological effects; Disease control; Field tests; Host plants; Host specificity; Inoculation; Leaves; Microstegium vimineum; pathogenicity; Plant diseases; Plant species; Plants; Plants (botany); Puccinia; rust disease; Rust fungi; Signs and symptoms; urediniospore; Urediniospores; Virulence; Weeds; China; pathogenicity; urediniospore; biological control; Microstegium vimineum; rust disease
• ISSN: 1526-498X; 1526-4998; 1526-4998
• DOI: 10.1002/ps.8180

BACKGROUND Microstegium vimineum (Trin.) A. Camus, commonly called stiltgrass, is a dominant weed in the United States and China. Although a lot of control approaches have been attempted, an economic, effective and practical measure has not been available to control the weed so far. RESULTS A serious rust disease of Microstegium vimineum was observed in three regions of Wenzhou city in China, from 2019 to 2021, with a disease incidence ranging from 82% to 97%. Typical rust disease symptoms on Microstegium vimineum were prominently visible during the early monsoon season (June–July), with chlorotic spots on the leaf surfaces. The morphological characterization of the strain WZ‐1 which was isolated from the diseased leaves was consistent with Puccinia polliniicola. The virulence tests showed that the average disease index of Microstegium vimineum plants could reach 35% at 10 days post‐inoculation. The host specificity of Puccinia polliniicola was tested on 64 plant species from 12 families and it did not cause any diseased symptoms on 24 major crops and 36 weeds, but slightly infected four gramineous weeds, Arthraxon hispidus, Polypogon fugax, Cynodon dactylon, and Microstegium ciliatum. However, newly‐produced urediniospores were not observed on the slightly infected plants. The urediniospores of strain WZ‐1 infected the Microstegium vimineum leaves by two main approaches: mycelium or appressorium invaded the stoma; and mycelium or appressorium directly invaded intercellular spaces. Field experiments showed that the rust disease naturally prevailed among Microstegium vimineum populations, causing severe rust disease symptoms on the leaf surface. The rust epidemic effectively controlled all of the target plants in the closed plot where the rust was released. CONCLUSION Puccinia polliniicola strain WZ‐1 has great potential to be used as a classical biological control agent against Microstegium vimineum. © 2024 Society of Chemical Industry. Puccinia polliniicola strain WZ‐1 has great potential to be used as a biological control agent against Microstegium vimineum.