Efficient method of determining tungro virus resistance in rice ( Oryza sativa L.)

To determine the level of rice seedlings with rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV), the seedlings were either mass inoculated with viruliferous green leafhopper (Glh), Nephotettix virescens, or individual seedlings were caged with the viruliferous Glh (forced i...

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Bibliographic Details
Published inCrop protection Vol. 10; no. 3; pp. 195 - 198
Main Authors Shahjahan, M., Zakri, A.H., Imbe, T., Jalani, B.S., Omura, T.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.06.1991
Subjects
ELISA
forced inoculation
mass inoculation
screening
Tungro virus
Tungro virus
screening
forced inoculation
mass inoculation
ELISA
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Summary:To determine the level of rice seedlings with rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV), the seedlings were either mass inoculated with viruliferous green leafhopper (Glh), Nephotettix virescens, or individual seedlings were caged with the viruliferous Glh (forced inoculation). The plant samples were analysed by enzyme-linked immunosorbent assay (ELISA) to detect the RTBV and RTSV particles, and by visual assessment. In mass inoculation 17.4% of the seedlings escaped viral infection compared with 1.4% for forced inoculation. A close correlation was found to exist between the assessment by ELISA and visual scoring.
ISSN:0261-2194
1873-6904
DOI:10.1016/0261-2194(91)90042-P