Quantitative collection of micro amounts of steroids from gas-liquid chromatography

A detailed study of the collection of micro amounts of steroids from GLC is presented. Collection is quantitative over a wide range of steroid concentrations (1–200 μg) when the argon effluent is condensed in a U-tube immersed in liquid nitrogen. It is also possible to collect steroids quantitativel...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 7; no. 2; pp. 135 - 146
Main Authors Brooks, S.C., Godefroi, V.C.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.01.1964
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Summary:A detailed study of the collection of micro amounts of steroids from GLC is presented. Collection is quantitative over a wide range of steroid concentrations (1–200 μg) when the argon effluent is condensed in a U-tube immersed in liquid nitrogen. It is also possible to collect steroids quantitatively from GLC in capillaries maintained at room temperature. By using a 1-mm i.d. glass tube as the receiver, the exact amount of collected steroid may be calculated from a suitable equation for a 1–100 μg range; or quantitative collection may be obtained over a limited range by employing a 0.6-mm i.d. capillary.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(64)90220-9