Agaricus bisporus mediated biosynthesis of copper nanoparticles and its biological effects: An in-vitro study

Biofabrication of Copper nanoparticles (CuNPs) using Agaricus bisporus aqueous extract and its potential in-vitro biological activities were attempted in the present work. The synthesized CuNPs was confirmed by absorption peak at 551 nm using UV–Vis spectroscopy. The crystalline properties and the b...

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Published inColloid and interface science communications Vol. 35; p. 100254
Main Authors Sriramulu, Mohana, Shanmugam, Sumathi, Ponnusamy, Vinoth Kumar
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.03.2020
Subjects
Agaricus bisporus
Anti-oxidant
Bactericidal
Copper nanoparticles
ROS production
Agaricus bisporus
Anti-oxidant
Bactericidal
ROS production
Copper nanoparticles
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Summary:Biofabrication of Copper nanoparticles (CuNPs) using Agaricus bisporus aqueous extract and its potential in-vitro biological activities were attempted in the present work. The synthesized CuNPs was confirmed by absorption peak at 551 nm using UV–Vis spectroscopy. The crystalline properties and the biofunctional groups involved in the reduction process were authenticated by XRD and FTIR spectroscopic techniques. Zeta potential value of −40 mV confirmed the stability of CuNPs. SEM and TEM analysis revealed agglomerated morphology and the presence of elemental Cu was confirmed by EDAX. In-vitro anti-bacterial assay demonstrated good bactericidal effects with more inhibitory action against Enterobacter aerogens (15 ± 0.08 mm). Anti-oxidant assays revealed potential scavenging effect against ABTS radical (82%); NO radical (76%) and DPPH radical (72%). Cytotoxic assay also revealed potential inhibitory effects against human colon cancer cell lines (SW620) with 60% cell viability recorded at 500 μg/ml concentration. Fluorescent microscopic observation of SW620 cells stained by DCFHDA and acridine orange dye displayed fragmentation, membrane loss and generation of ROS apoptotic features. [Display omitted] •The first report on Agaricus bisporus mediated copper nanoparticles•Excellent antibacterial activity against gram negative bacteria.•Rapid scavenging potential against ABTS free radicals.•Enhanced cytotoxic effect against SW620 cancer cell lines.
ISSN:2215-0382
2215-0382
DOI:10.1016/j.colcom.2020.100254