Preparation and evaluation a mixed-mode stationary phase with imidazolium and carboxyl group for high performance liquid chromatography

• Published in: Microchemical journal Vol. 150; p. 104131
• Main Authors: Xian, Hong, Peng, Huanjun, Wang, Xiang, Long, Dengying, Ni, Ranxi, Chen, Jun, Li, Shiyu, Zhang, Zhongying, Peng, Jingdong
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2019
• Subjects: Click chemistry; Hydrophilic/reversed phase/ion exchange; Ionic liquid; Linear solvation energy equation; Mixed-mode chromatography stationary phase; Linear solvation energy equation; Click chemistry; Mixed-mode chromatography stationary phase; Hydrophilic/reversed phase/ion exchange; Ionic liquid
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2019.104131

A novel stationary phase based on ionic liquid was prepared through thiol-ene click chemistry by functionalizing imidazolium and a carboxyl group for the mixed-mode liquid chromatography. The prepared material was characterized by elemental analysis, thermogravimetric analysis and FT-IR spectroscopy. The retention behavior of the stationary phase under different conditions (water content, pH, buffer concentration in the mobile phase and column temperature) was investigated. The interactions which occurred in the separation system were quantitatively studied by linear solvation energy equation and compared with two commercial columns. Moreover, the polar nucleosides and nucleobases, sulfonamides and inorganic anions were separated on a single mixed-mode column and exhibited high efficiency and flexible selectivity. In brief, the new stationary phase could be adapted to separate complex compounds with varying properties. •A novel mixed-mode stationary phase based on ionic liquid for high performance liquid chromatography was prepared by thiol-ene click chemistry.•The retention mechanism was investigated under different conditions (water content, pH, buffer concentration and column temperature).•The interactions which occurred in the separation system were analyzed by linear solvation energy equation and compared with two commercial columns.•Different mixtures were separated under HILIC/RPLC/IEC mode, showing satisfactory performance and different selectivity with commercial column.