An UiO-66/P-L-histidine composite film fabricated by electropolymerization and electrodeposition for sensing biomarker 4-nitroquinoline N-oxide

• Published in: Microchemical journal Vol. 172; p. 106925
• Main Authors: Niu, Yuanyuan, Zhou, Jianfeng, Lai, Haohong, Zhou, Qing, Wang, Shumei, Zhai, Haiyun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2022
• Subjects: 4-nitroquinoline N-oxide; Differential pulse voltammetry; Electrochemical sensor; Metal organic framework UiO-66; Poly L-histidine; Electrochemical sensor; Metal organic framework UiO-66; 4-nitroquinoline N-oxide; Poly L-histidine; Differential pulse voltammetry
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2021.106925

[Display omitted] •A facile, reliable, low-cost, and sensitive electrochemically glassy carbon electrode modified with Zr4+-based metal-organic framework UiO-66 and poly L-histidine (P-L-his) composite was constructed and characterized.•Unlike most modification methods, UiO-66 was modified on the surface of electrode by electrodeposition in this study. Compared with drop coating, electrodeposition proceeds efficiently under mild conditions and often requires shorter time.•The fabricated sensor exhibits excellent electrocatalytic activities for the analysis of 4-nitroquinoline N-oxide (4-NQO) and was successfully applied in the human blood samples.•Under optimum conditions, the modified electrode exhibits good analytical performance with considerable sensitivity, stability, anti-jamming capability and reproducibility. A sensitive and selective electrochemical sensor for sensing 4-nitroquinoline N-oxide (4-NQO) by differential pulse voltammetry was designed. Poly L-histidine (P-L-his) composite and metal-organic framework UiO-66 (Zr) modified electrode was used as the working electrode which had large specific surface area, mesoporous structure and excellent conductivity. Unlike most modification methods, UiO-66 was electrodeposited in this study. Compared with drop coating, electrodeposition proceeds efficiently under mild conditions and often requires shorter time. FTIR, EDX, TEM, SEM and electrochemical assay were used to characterize the prepared materials and modified electrodes. After that, some experimental conditions like electro-polymerization time, deposition time, pH and scan rate were also improved and optimized separately. At the UiO-66/P-L-his-modified glassy carbon electrode, a good linear relationship between 4-NQO concentration and its peak current was obtained under the best conditions in a wide range from 0.2 to 50.0 μM, and the limit of detection (S/N = 3) was 66.7 nM. Moreover, multiple experiments elucidated that the as-synthesized sensor could be applied to detect 4-NQO in actual samples, and the recoveries results obtained were satisfactory.