Dual-site mitochondria-targeted fluorescent probe for simultaneous distinguishing detection of hypochlorite and SO2 derivatives in real water samples and bioimaging

• Published in: Dyes and pigments Vol. 207; p. 110706
• Main Authors: Lan, Jinshuai, Wang, Yu, Qin, Yanhong, Li, Zhe, Zeng, Ruifeng, Liu, Li, Chen, Lixia, Yang, Kaixu, Ding, Yue, Zhang, Tong
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.11.2022
• Subjects: Dual-site; Fluorescent probe; Hypochlorite; Mitochondria; SO2 derivatives; Mitochondria; SO2 derivatives; Dual-site; Hypochlorite; Fluorescent probe
• ISSN: 0143-7208; 1873-3743
• DOI: 10.1016/j.dyepig.2022.110706

SO2 derivatives and hypochlorite are ubiquitous in the environment and organisms, but excessive SO2 derivatives or ClO− can cause environmental pollution and serious diseases. Therefore, fluorescent probes to simultaneously test SO2 derivatives and ClO−in vitro and vivo are greatly needed. However, reported fluorescent probes with single recognition site for SO2 derivatives and ClO− detection have a competition and cross-talk, making the test inaccurate. So a novel dual-site mitochondria-targeted fluorescent probe (probe BEDB) was developed and could respectively response to SO2 derivatives and ClO− by distinct fluorescence with desirable properties, such as good selectivity, high sensibility and rapid response. The sensing mechanisms of SO32− and ClO− by probe BEDB were confirmed as nucleophilic addition and oxidation respectively by HPLC and HR-MS. Probe BEDB was used to accurately detect SO32− or ClO− in real samples, including river water, tap water and Tremella. Based on colorimetric methods, the fast-testing tube of probe BEDB could quickly and easily test ClO− or SO2 derivatives in real samples. Moreover, the probe was also effectively applicable to image SO2 derivatives and ClO− in living cells and zebrafish with mitochondria-targeted, and a crosstalk between SO2 derivatives and ClO− in the oxidative stress of acute liver injury models was also verified. Therefore, the dual-site fluorescent probe is a potent molecular tool for simultaneous distinguishing detection of SO2 derivatives and ClO−. A novel dual-site mitochondria-targeted fluorescent probe used to accurately detect SO32− or ClO− in real samples and bioimaging. [Display omitted] •A new dual-site fluorescent probe BEDB for simultaneous detection of ClO− and SO2 derivatives was synthesized.•Probe BEDB displayed high selectivity and sensitivity to SO32− and ClO− with separate fluorescence signals in aqueous solution.•Probe BEDB with mitochondria-targeted was applicable for SO2 derivatives and ClO− detection in real samples and bioimaging.