Scalable synthesis of Ce-MOF derived CeO/C Hierarchical: Efficient electrochemical sensing of uric acid as potential biomarker in acute myeloid leukaemia patients

• Published in: Microchemical journal Vol. 181; p. 107808
• Main Authors: Saeed, Ummama, Fatima, Batool, Hussain, Dilshad, Imran, Muhammad, Jamil, Maryam, Naeem Ashiq, Muhammad, Najam-ul-Haq, Muhammad
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2022
• Subjects: Acute Myeloid Leukemia (AML); Cyclic Voltammetry (CV); Differential Pulse Voltammetry (DPV); Electrochemical sensing; Uric acid; Ce-MOF; PBS; AML; UV; Differential Pulse Voltammetry (DPV); GCE; AFM; DPV; XRD; Uric acid; LSV; TGA; BET; Cyclic Voltammetry (CV); CV; FTIR; EDX; Electrochemical sensing; SEM; TEM; Acute Myeloid Leukemia (AML)
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2022.107808

[Display omitted] •Nano-Sized Morphology of Ce-MOF with pure crystallinity.•Electrochemical detection of Uric acid as Potential Biomarker of AML.•Positive & Negative Correlation Analysis of Uric acid with other significant biological molecules.•Synthesis of Ce-MOF having significant electrocatalytic activity towards Uric acid.•Selectivity & Stability of Ce-MOF towards Uric acid.•Real samples monitoring of Uric acid in AML & stepping towards Personalized Medicine in future. Uric acid is an end product of purine metabolic pathway. Any alteration in its metabolism leads to diseases including acute myeloid leukemia (AML). In AML patients, uric acid levels are the potential predictor of disease aggressiveness. Various detection techniques have been used for uric acid analysis from biological samples. Among these, electrochemical sensing is specific, rapid, automatic, and economical. An electrical impulse detects in the form of redox peaks obtained via cyclic voltammetry and differential pulse voltammetry. Herein, a Ce-MOF sensor is developed for uric acid determination through CV and DPV. Ce-MOF is synthesized in a single-step and calcined at 400 °C. Sensing parameters like pH, concentration, scan rate, and effect of interferences are optimized. Linearity, limit of detection (LOD), and limit of quantification (LOQ) obtained from CV and DPV are 0.9950 M, 0.26 × 10−3 M, and 8.7 × 10−3 M, and 0.966 × 10−6M, 0.9 × 10−6M, and 9.68 × 10−6M, respectively. The stability of Ce-MOF-400 °C modified electrode is measured by 100 CV cycles. Ce-MOF-400 °C quantitatively determines uric acid from serum samples of Acute Myeloid Leukemia (AML) patients. The normal distribution of biological parameters data is determined by Shapiro-Wilk analysis. Positive and negative correlations of uric acid with other biological parameters are observed by paired t-test.