Identification of Resistance QTLs to Black Leaf Streak Disease (Due to Pseudocercospora fijiensis) in Diploid Bananas (Musa acuminata)

• Published in: Horticulturae Vol. 10; no. 6; p. 608
• Main Authors: Carreel, Françoise, Martin, Guillaume, Ravel, Sébastien, Roussel, Véronique, Pages, Christine, Habas, Rémy, Cantagrel, Théo, Guiougou, Chantal, Delos, Jean-Marie, Hervouet, Catherine, Mournet, Pierre, D’Hont, Angélique, Yahiaoui, Nabila, Salmon, Frédéric
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 07.06.2024; MDPI
• Subjects: Agricultural sciences; banana; Bananas; Black Leaf Streak Disease; black sigatoka; Chromosome translocations; Chromosomes; Controlled conditions; Cultivars; Diploids; Disease; Disease resistance; Environmental impact; Epidemiology; Fertility; Flowers & plants; Fruits; Fungicides; Genetics; Genomes; Hybrids; Inoculation; Leaf streak; Leaves; Life Sciences; Marker-assisted selection; Musa; Phytopathology and phytopharmacy; Plant breeding; Plant diseases; Plants genetics; Progeny; Pseudocercospora fijiensis; QTL; Quantitative trait loci; Recombination; Sciences and technics of agriculture; Seeds; Soils; Vegetal Biology; Honduras; Guadeloupe; India; Kolkata India; banana; QTL; Musa; Pseudocercospora fijiensis; Black Leaf Streak Disease; black sigatoka
• ISSN: 2311-7524; 2311-7524
• DOI: 10.3390/horticulturae10060608

Black Leaf Streak Disease (BLSD), caused by the fungus Pseudocercospora fijiensis, is a recent pandemic and the most economically and environmentally important leaf disease of banana. To assist breeding of varieties with durable resistance to the rapidly evolving P. fijiensis, we used a diploid genitor ‘IDN 110’ with partial resistance to BLSD to search for QTLs. We assessed diploid progeny of 73 hybrids between ‘IDN 110’ and the diploid cultivar ‘Khai Nai On’, which is susceptible to BLSD. Hybrids were phenotyped with artificial inoculation under controlled conditions. This method allowed us to focus on resistance in the early stages of the interaction already identified as strongly influencing BLSD epidemiology. Progeny were genotyped by sequencing. As both parents are heterozygous for large reciprocal translocations, the distribution of recombination was assessed and revealed regions with low recombination rates. Fourteen non-overlapping QTLs of resistance to BLSD were identified of which four main QTLs from the ‘IDN110‘ parent, located on chromosomes 06, 07, 08, and 09, were shown to be of interest for marker-assisted selection. Genes that underline those four QTLs are discussed in the light of previous literature.