Ultrasensitive detection of alpha-synuclein oligomer using a PolyD-glucosamine/gold nanoparticle/carbon-based nanomaterials modified electrochemical immunosensor in human plasma

• Published in: Microchemical journal Vol. 158; p. 105195
• Main Authors: Tao, Dan, Gu, Yingying, Song, Shizhen, Nguyen, Emily P., Cheng, Jing, Yuan, Qiong, Pan, Hongzhi, Jaffrezic-Renault, Nicole, Guo, Zhenzhong
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2020
• Subjects: Alpha-synuclein oligomer; Electrochemical immunosensor; Parkinson’s disease; Poly(D-glucosamine)/gold nanoparticles/ multi-walled carbon nanotubes/reduced graphene oxide; Signal amplification; Electrochemical immunosensor; Poly(D-glucosamine)/gold nanoparticles/ multi-walled carbon nanotubes/reduced graphene oxide; Alpha-synuclein oligomer; Parkinson’s disease; Signal amplification
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2020.105195

•The PDG/AuNPs/MWCNTs/rGO immunosensor was successfully constructed.•This sensor exhibited highly sensitive and stability and LOD was 0.03 fM.•This work has significance for the early diagnosis of PD. To discover the early stage Parkinson’s disease (PD) patients and provide treatment ideas, an ultrasensitive poly (D-glucosamine)/gold nanoparticles/ multi-walled carbon nanotubes/reduced graphene oxide (PDG/AuNPs/MWCNTs/rGO) modified immunosensor was proposed to detect alpha-synuclein oligomer (α-syno), which is the early biomarker in PD. As signal amplification materials, the PDG/AuNPs/ MWCNTs/rGO nanocomplex was immobilized on the glassy carbon electrode surface, and anti-α-syno antibody that specifically recognizes and binds α-syno is then grafted. The immunosensor revealed a linear response between 0.05 fM and 500.00 fM, using square wave voltammetry (SWV), and a detection limit of 0.03fM was found. The immunosensor with excellent sensitivity, selectivity and stability has been applied to the analysis of α-syno in human plasma samples.