Multi-Omics Profiles of Small Intestine Organoids in Reaction to Breast Milk and Different Infant Formula Preparations

• Published in: Nutrients Vol. 16; no. 17; p. 2951
• Main Authors: Wang, Xianli, Yang, Shangzhi, Zheng, Chengdong, Huang, Chenxuan, Yao, Haiyang, Guo, Zimo, Wu, Yilun, Wang, Zening, Wu, Zhenyang, Ge, Ruihong, Cheng, Wei, Yan, Yuanyuan, Jiang, Shilong, Sun, Jianguo, Li, Xiaoguang, Xie, Qinggang, Wang, Hui
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.09.2024
• Subjects: Baby foods; Breastfeeding & lactation; Female; growth development; Humans; Infant; Infant Formula; infant formulas; Infant Nutritional Physiological Phenomena; Infant, Newborn; Intestine, Small - metabolism; Lactation; metabolomics; Metabolomics - methods; Milk, Human - chemistry; Multiomics; Nutrition; organoids; Organoids - metabolism; small intestine; Stem cells; Transcriptome; United States > US; China; small intestine; infant formulas; metabolomics; organoids; transcriptome; growth development
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16172951

Ensuring optimal infant nutrition is crucial for the health and development of children. Many infants aged 0-6 months are fed with infant formula rather than breast milk. Research on cancer cell lines and animal models is limited to examining the nutrition effects of formula and breast milk, as it does not comprehensively consider absorption, metabolism, and the health and social determinants of the infant and its physiology. Our study utilized small intestine organoids induced from human embryo stem cell (ESC) to compare the nutritional effects of breast milk from five donors during their postpartum lactation period of 1-6 months and three types of Stage 1 infant formulae from regular retail stores. Using transcriptomics and untargeted metabolomics approaches, we focused on the differences such as cell growth and development, cell junctions, and extracellular matrix. We also analyzed the roles of pathways including AMPK, Hippo, and Wnt, and identified key genes such as ALPI, SMAD3, TJP1, and WWTR1 for small intestine development. Through observational and in-vitro analysis, our study demonstrates ESC-derived organoids might be a promising model for exploring nutritional effects and underlying mechanisms.