Quinolizinium-based tunable pH fluorescent probes for imaging in live cells

A new class of quinolizinium-based tunable pH fluorescent probes for cell imaging was developed. The probes showed turn “On” fluorescence response upon acidification brought by a modulation of photoinduced electron transfer (PET) from the N,N-dialkyl aniline substituent to the quinolizinium core ske...

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Bibliographic Details
Published inDyes and pigments Vol. 205; p. 110541
Main Authors Sit, Hoi-Yi, Deng, Jie-Ren, Chan, Wing-Cheung, Ko, Ben Chi-Bun, Wong, Man-Kin
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.09.2022
Subjects
Cellular imaging
pH probe
Photo-induced electron transfer
Quinolizinium
Quinolizinium
Cellular imaging
Photo-induced electron transfer
pH probe
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Summary:A new class of quinolizinium-based tunable pH fluorescent probes for cell imaging was developed. The probes showed turn “On” fluorescence response upon acidification brought by a modulation of photoinduced electron transfer (PET) from the N,N-dialkyl aniline substituent to the quinolizinium core skeleton. These novel PET-based pH fluorescent probes displayed tunable emission properties in between 478 and 509 nm. Also, the probes exhibited tunable pH responsiveness with pKa ranging from <2.1 to 6.1 contributed by the steric effect of N,N-dialkyl anilines. They were successfully applied to in vivo pH sensing in lysosome in live cells. [Display omitted] •A new class of PET-based pH fluorescent probes was developed based on a quinolizinium core skeleton with an aniline moiety.•These novel PET-based pH fluorescent probes exhibited tunable emission properties and pH responsiveness.•The steric effect of N,N-dialkyl anilines resulted in different pKa values of the pH fluorescent probes.•They were successfully applied to pH sensing in vivo in lysosome of live cells.
ISSN:0143-7208
1873-3743
DOI:10.1016/j.dyepig.2022.110541