A simple one-step transferred sample preparation for effective purification and extraction of auramine O in bean product by combining air-assisted ionic liquid-based dispersive liquid-liquid microextraction

• Published in: Microchemical journal Vol. 159; p. 105571
• Main Authors: Zhang, Yaqi, Sun, Yaming, Yang, Zhen, Jin, Shaofeng, Gao, Li, He, Lijun, Jiang, Xiuming
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2020
• Subjects: Air assistance; Aurmine O; Bean product; Ionic liquid-based dispersive liquid-liquid microextraction; One-step transfer; Ionic liquid-based dispersive liquid-liquid microextraction; Bean product; Air assistance; Aurmine O; One-step transfer
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2020.105571

•A simple AA-IL-DLLME was used to extract AO from bean products.•The whole procedure needed only one transfer operation and 1.5 mL of organic solvent.•Air assistance promoted the rapid and good dispersion of IL in low volume of dispersant.•IL with vinyl group provided high ER for AO via multiple-interaction. A one-step transferred sample preparation was developed for the direct purification and extraction of auramine O (AO) in solid bean products by combining air-assisted ionic liquid-based dispersive liquid-liquid microextraction (AA-IL-DLLME). The amount of AO was then determined using high-performance liquid chromatography-ultraviolet/visible detection. Herein, acidified extractant was employed to extract AO from solid samples and served as dispersant of AA-IL-DLLME simultaneously, which simplified the procedure and achieved the one-step transferred operation. The air assistance in IL-DLLME facilitated the rapid and sufficient dispersion of IL in low volume of dispersant, decreasing the consumption of organic solvent and enhancing the extraction efficiency. The high extraction recovery of nearly 90% was ascribed to the possible hydrophobic and π-π interactions between IL and AO. Parameters affecting extraction efficiency, including type and volume of extractant and IL, vortex and centrifugation time etc., were investigated in detail. The limit of detection (LOD, S/N = 3) and limit of quantification (LOQ, S/N = 10) were 0.01 μg g−1 and 0.04 μg g−1, respectively. The relative standard deviations (RSDs, n = 5) for intra-day and inter-day were 2.7%–7.4% and 4.3%–8.1%, respectively. The applicability of the proposed technique was demonstrated by analyzing various bean products with satisfactory relative recoveries of 83.5%–104.8% and RSDs (n = 3) of 1.8%–6.2%.