A simple extract of Leucaena leucocephala (Lam.) de Wit leaf containing mimosine as a natural color reagent for iron determination

[Display omitted] •Simple aqueous extract of L. leucocephala leaf contains mimosine (hydroxypyridinone).•Use as natural color reagent for iron assays that is different from so far reported.•The performance similarly to that of commercial mimosine but with much less cost.•Demonstrated for real blood...

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Bibliographic Details
Published inMicrochemical journal Vol. 162; p. 105860
Main Authors Kotchabhakdi, Narong, Seanjum, Chalermpong, Kiwfo, Kanokwan, Grudpan, Kate
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.03.2021
Subjects
Hydroxypyridinone
Iron
Leucaena leucocephala
Mimosine
Natural reagent
Mimosine
Natural reagent
Iron
Hydroxypyridinone
Leucaena leucocephala
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Summary:[Display omitted] •Simple aqueous extract of L. leucocephala leaf contains mimosine (hydroxypyridinone).•Use as natural color reagent for iron assays that is different from so far reported.•The performance similarly to that of commercial mimosine but with much less cost.•Demonstrated for real blood tonic samples.•Offering an alternative green chemical analysis. A simple extract of Leucaena leucocephala (Lam.) de Wit leaf containing mimosine is proposed as a natural color reagent for the determination of iron. The extract exhibited the formation of Fe(III) with mimosine similarly to that of the commercially available mimosine. A linear calibration: A460 = 6.8 × 10−2 [Fe(III)] + 8.2 × 10−2, R2 = 0.99 was obtained for 0–10 mg.L−1, with LOD (3σ), LOQ (10σ) and %RSD being 0.2 mg.L−1, 0.7 mg.L−1 and less than 5, respectively. The proposed procedure was demonstrated for real samples of blood tonic. The results agreed with that of the FAAS method.
ISSN:0026-265X
1095-9149
DOI:10.1016/j.microc.2020.105860