A Zn-Al-Zr layered double hydroxide/graphene oxide nanocomposite enables rapid photocatalytic removal of kanamycin-resistance bacteria and genes via nano-confinement effects

• Published in: Applied catalysis. B, Environmental Vol. 350; p. 123922
• Main Authors: Yu, Miao, Liu, Dawei, Wang, Lichao, Xia, Jing, Ren, Jianhui, Fan, Yuqiu, Zhu, Xiaofeng, Wang, Jun, Xiong, Kun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05.08.2024
• Subjects: Antibiotic-resistance; Differential Proteomics; Nano-confinement Effects; Photocatalysis; Wastewater Treatment; Nano-confinement Effects; Differential Proteomics; Antibiotic-resistance; Photocatalysis; Wastewater Treatment
• ISSN: 0926-3373; 1873-3883
• DOI: 10.1016/j.apcatb.2024.123922

For efficient removal of the antibiotic-resistance bacteria and antibiotic-resistance genes, zirconium-doped zinc-aluminum layered double hydroxide/graphene oxide (Zn-Al-Zr LDHs/GO) nanocomposite has been developed. The Zn-Al-Zr LDHs/GO shows outstanding photocatalytic sterilization, inactivating kanamycin-resistance E. coli (6.53 log10 CFU/mL) under the full-wavelength light irradiation within 50 minutes. Owing to the 1O2 formation, it greatly disrupts the respiratory chain of the kanamycin-resistance E. coli, thereby leading to a declined yield of adenosine triphosphate, down-regulated expressions of the DNA ligase and polymerase proteins, as well as completely inhibited expressions of the DNA repair proteins. Meanwhile, the strong adsorption capability of the Zn-Al-Zr LDHs/GO towards phosphorus endows it with special nano-confinement effects for ARGs. It results in significantly enriched local concentration of the kanamycin-resistance genes on the Zn-Al-Zr LDHs/GO, consequently enhanced removal ability towards the kanamycin-resistance genes (4.70 log10 copies/mL in 3 hours). This work provides a new strategy for effectively removing the ARB and ARGs. [Display omitted] •Zn-Al-Zr LDHs/GO binds e-ARGs through the nano-confinement effects.•Zn-Al-Zr LDHs/GO removes ARB with different concentrations in 60 min by photocatalytic.•ATP yield in ARB decreased and DNA replication was blocked by 1O2.•Zn-Al-Zr LDHs/GO has stable material structure and photocatalytic activity.