A bichromophoric squaraine based NIR fluorescent probe for G-quadruplex in living cells
The polymorphic formation of G-quadruplex brings more attentions, because changes of formation sites affect many physiological processes, like telomere maintenance, transcription and DNA replication. These processes are highly susceptible to ROS-induced oxidative damage. In this work, bichromophoric...
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Published in | Dyes and pigments Vol. 227; p. 112159 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
01.08.2024
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Subjects | |
Online Access | Get full text |
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Summary: | The polymorphic formation of G-quadruplex brings more attentions, because changes of formation sites affect many physiological processes, like telomere maintenance, transcription and DNA replication. These processes are highly susceptible to ROS-induced oxidative damage. In this work, bichromophoric cores of symmetric squaraine probe SQ linked with ether chain is conducive to the recognition of G-quadruplex, based on the mechanism of intra or intermolecular “aggregation-disaggregation”. Several interaction behaviors, such as self-aggregation, binding affinity, stoichiometric ratio, were fully investigated via spectrum titration, circular dichroism, fluorescent intercalator displacement assay, and molecular docking. Furthermore, tracking G-quadruplex distribution in the cytoplasm was monitored by SQ during the oxidative stress enhancement. Interestingly, the G-quadruplex levels in the cytoplasm of cancer cells were slightly higher than that of normal cells to deal with oxidation-reduction imbalance. Overall, the lower detection limit of SQ for parallel G-quadruplex like pu27 is as low as 7.2 nM; and the complex of SQ and G-quadruplex will not change the topological structure of G-quadruplex. It has good sensitive and stability for parallel type of G-quadruplex. It is a potential tool for bioimaging of G-quadruplex in the cytoplasm.
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•A symmetry biochromophoric squaraine probe targeting to G-quadruplex was synthesized.•SQ can discriminate G-quadruplex from single and double stranded DNA.•H-aggregates could be disassociated by parallel G-quadruplexes via a turn-on protocol.•A new supermolecular assembly was docked as a sandwich-like model.•SQ can track G-quadruplex in the cytoplasm under the oxidative stress. |
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ISSN: | 0143-7208 1873-3743 |
DOI: | 10.1016/j.dyepig.2024.112159 |