A comparison of the biochemical and physiological properties of a polygalacturonase from two races of Colletotrichum lindemuthianum

• Published in: Physiological and molecular plant pathology Vol. 37; no. 3; pp. 193 - 206
• Main Authors: Keon, John P.R., Waksman, Gabriel, Bailey, John A.
• Format: Journal Article
• Language: English
• Published: London Elsevier India Pvt Ltd 1990; Elsevier
• Subjects: Agronomy. Soil science and plant productions; amino acid sequences; Biological and medical sciences; characterization; Colletotrichum lindemuthianum; cultivars; enzyme activity; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; host specificity; pathophysiology; pathotypes; Pest resistance; Phaseolus vulgaris; Plant pathogens; polygalacturonase; varietal resistance; Varietal selection. Specialized plant breeding, plant breeding aims; Mycosis; Host specificity; Biochemistry; Phytopathogen; Polygalacturonase; Molecular weight; Fungus resistance; Fungi; Infection; Specific activity; Leguminosae; Enzymatic activity; Vegetable crop; Phaseolus vulgaris; Dicotyledones; Aminoacid; Angiospermae; Colletotrichum lindemuthianum; Spermatophyta; Physiology; Fungi Imperfecti; Thallophyta; Cultivar
• ISSN: 0885-5765; 1096-1178
• DOI: 10.1016/0885-5765(90)90011-L

An endo-glycolytic polygalacturonase [EC 3·2·1·15] was purified to homogeneity from culture filtrates of Colletotrichum lindemuthianum races beta and gamma. The molecular weights (c. 39000), pls (9·4), N-terminal amino acid sequences, amino acid compositions, specific activities and the substrate utilization of the enzymes were indistinguishable. Hypocotyl tissues from cultivars of Phaseolus vulgaris, that differed in their resistance and susceptibility to races beta and gamma of C. lindemuthianum, were all rapidly macerated and killed by endopolygalacturonase from both races of fungus. These data suggest that the biochemical and physiological properties of the endopolygaiacturonase produced by C. lindemuthianum cannot explain the cultivar specificity exhibited by different races of this pathogen.